Phytochrome-mediated Induction of Phenylalanine Ammonia-Lyase in Mustard Seedlings

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Phytochrome-mediated Induction of Phenylalanine Ammonia-Lyase in Mustard Seedlings: A Contribution to Eliminate Some Misconceptions.

The present report shows that the effect of red and far red light on the synthesis of phenylalanine ammonia-lyase can be ascribed to the action of phytochrome Pfr. This is true not only for short term irradiation but also for the action of continuous far red light. In the latter case, the model worked out by Hartmann implicating some excited species of phytochrome has to be taken into account.

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The molecular mechanism of enzyme (phenylalanine ammonia-lyase; EC 4.3.1.5) formation mediated by phytochrome in mustard seedlings was investigated by labeling the enzyme with deuterium followed by high resolution CsCl density gradient analysis. A favorable relationship between induced rise of activity and turnover of this short-lived enzyme (half-life 3-4 hr) was achieved by labeling pre-irrad...

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Ethylene-enhanced Synthesis of Phenylalanine Ammonia-Lyase in Pea Seedlings.

The effect of ethylene on the development of phenylalanine ammonia-lyase activity in segments excised from the epicotyl apex of pea seedling was studied. Although there was some increase in phenylalanine ammonia-lyase activity in segments not treated with ethylene, a marked increase in phenylalanine ammonia-lyase activity occurred in ethylene-treated tissues during the incubation. The induction...

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Yeast Phenylalanine Ammonia-lyase

I?henyialanine ammonia-lyase from the yeast Rhodotorula gluiinis was purified by salt fractionations and Sephadex chromatography. Density gradient centrifugation and Sephadex chromatography indicated its molecular weight to be about 275,000. Enzymatic deamination of several ring-substituted phenylalanine analogues and n-phenylalanine was studied. While cinnamic acid, a product of deamination, a...

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Red and far red effects on phenylalanine ammonia-lyase in raphanus and sinapis seedlings do not correlate with phytochrome spectrophotometry.

In seedlings of Raphanus sativus (radish) and Sinapis alba (mustard), irradiation for 6 hours with far red light significantly increases the extractable activity of phenylalanine ammonia-lyase by the end of the light period. A schedule of 10 minutes red light-110 minutes darkness-10 minutes red-110 minutes darkness-10 minutes red-110 minutes darkness has no effect as compared to dark controls. ...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1972

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.49.1.8